Fig. 1.

MyoVc and Spire1 localize exclusively to mature WPB. HUVEC were transfected with un-targeting siRNA (siControl) or siRNA directed against Rab27a and MyoVc-GFP or Spire1-GFP (cyan) as indicated. Cells were fixed and stained for VWF as general WPB marker (yellow) or Rab27a as marker for mature WPB using the respective antibodies (magenta). A Shows merged pictures of MyoVc-GFP-expressing cells with peri-nuclear and peripheral regions marked by boxes. Boxed regions are enlarged in B. DAPI stainings are shown in blue. Scale bars: 10 µm. C Shows cells expressing Spire1-GFP. Peri-nuclear and peripheral regions are marked by boxes. Boxed regions are enlarged in D. Scale bars: 10 µm