Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 27;79(2):96. doi: 10.1007/s00018-021-04108-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — The SB domain of Spire1 is sufficient for mediating recruitment to WPB. A Depicts the domain structure of Spire 1 consisting of an actin-binding (WH2) and formin recruiting (KIND) N-terminal part. The C-terminal part comprises a Spire box domain (SB), a FYVE-type zinc finger and a H2 sequence similar to Slp/Slac proteins. B–E HUVEC were transfected with different GFP-tagged Spire1 truncation mutants (yellow) and co-stained for VWF (magenta). DAPI stainings are shown in cyan. Scale bars: 10 µm. The boxed areas are shown enlarged as a merge (upper picture) and as single channels (lower pictures, VWF and Spire mutants) next to the overview picture showing the whole cell. B Spire1 amino acid (aa) 388–742 (GTBM-SB-FYVE-H2, Spire1Tail). C Spire1 aa 525–742 (SB-FYVE-H2). D Spire1 aa 525–697 (SB-FYVE). E Spire1 aa 525–572 (SB)