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. 2022 Jan 27;79(2):96. doi: 10.1007/s00018-021-04108-x

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Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Spire1 interacts with MyoVc. Spire1-GFP or an empty GFP vector (GFP) were expressed ectopically in HUVEC. Cell lysates were directly analyzed by Western blot (Input) or further processed using GFP-trap pull-downs. GFP-trap-bound proteins (pull-down, PD) and the supernatant (SN) of the GFP-trap samples were also analyzed by Western blots using antibodies directed against GFP or MyoVc as indicated