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. 2022 Jan 27;79(2):96. doi: 10.1007/s00018-021-04108-x

Fig. 7.

Fig. 7

Depletion of either MyoVc or Spire1 reduces the number of actin rings at WPB fusion sites. HUVEC were transfected with siRNA (Control, MyoVc- or Spire1-targeting), Lifeact-GFP and VWF-RFP constructs. 24 h later, life-cell imaging was started and cells were stimulated with histamine (500 µM). Live-cell images were then analyzed by determining the number of WPB before and 5 min after stimulation, and by counting the amount of WPB fusion events accompanied by an actin ring. Shown are box-and-whisker plots depicting the absolute number of actin rings, the number of actin rings normalized to the total number of WPB per cell and the number of actin rings normalized to the number of fully fused WPB. siCtrl: n = 50 cells. siMyoVc: n = 30 cells. siSpire1: n = 50 cells. Statistics employed Kruskal–Wallis and Bonferroni-corrected post hoc tests