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. 2022 Jan 27;12:1473. doi: 10.1038/s41598-022-05176-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical staining of ALDH1A1, CD133, and mutant p53 of samples from lung adenocarcinoma patients. ALDH1A1 staining intensity was rated as weak (1+), moderate (2+), or strong (3+) and multiplied by the percentage of positive cells. ALDH1A1 score = (% of cells of intensity 1 × 1) + (% of cells of intensity 2 × 2) + (% of cells of intensity 3 × 3). The total scores were categorized as follows: 0–100 = grade 1; 101–200 = grade 2; and 201–300 = grade 3. Grade 2 or 3 tumors were considered positive for ALDH1A1. (A) ALDH1A1 score = 0 (intensity 0 × 0% positive cells). (B) ALDH1A1 score = 220 (intensity 1 × 20% positive cells + intensity 2 × 30% positive cells + intensity 3 × 50% positive cells). The percentage of CD133- and mutant p53-positive cells was graded as 0%–100%. CD133 positivity was defined as staining of more than 20% of the tumor cells (negative: ≤ 20%; positive: > 20%). (C) CD133-positive cells were 0%, negative. (D) CD133-positive cells were 100%, positive. (E) Mutant p53-positive cells were 0%, negative. (F) Mutant p53-positive cells were 100%, positive.