Figure 1.
Cell yield and viability. (a) Viability of cells is independent of volume processed for 1 mL volumes (squares) and 9 mL volumes (circles). F1,44 = 0.67, p = 0.42 for volume; F1,44 = 0.04, p = 0.84 for injury status; n = 10 controls (white symbols); n = 14 stroke patients (red/blue symbols). (b) Significant viability loss occurred during the cryopreservation process (clear bars) compared to viability prior to banking (hatched bars). * p < 0.05; **** p < 0.0001; n = 12 controls (clear circles), n = 16 stroke (filled circles, systemic; squares, intracranial), n = 12 cerebrovascular disease patients (triangles). One intracranial sample was unreadable using the Nexcelom Cellometer after recovery from cryopreservation but did have viable cells by flow cytometry. (c) Correlation between last known normal and % viability in intracranial blood at time of collection does not show an effect of time to mechanical thrombectomy on leukocyte yield; F1,11 = 1.81, p = 0.21. r2 = 0.14.