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. 2022 Jan 27;13:553. doi: 10.1038/s41467-022-28220-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Enantioselective differential chiral contrast (EDCC) CPL-LSCM of Eu:L1 (30 µM, 14 h loading, ×63 1.4 NA oil objective, 96 × 96 µm FOV, 100 avg., 790 nm axial section) in live mouse skin fibroblast (NIH 3T3) cells, showing enantioselective localisation to the lysosome (Δ-Eu:L1) and mitochondria (Λ-Eu:L1). Top row Δ-Eu:L1. A Total Europium emission (λ_ex = 355 nm, 20 mW, λ_em = 589–720 nm). B, C Left and Right CPL channel (λ_ex = 355 nm, λ_em = 589–599 nm), respectively. D Left-handed EDCC image (left CPL—right CPL) highlighting enantioselective predominantly lysosomal localisation. E Transmission image. Bottom row Λ-Eu:L1. F, G, H, J as per (A, B, C, E). I Left-handed EDCC image (left CPL—right CPL) highlighting enantioselective predominantly mitochondrial localisation. Scale bars = 20 µm, numbers in red are avg. Eight-bit pixel intensity values for each image region.