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. 2022 Jan 27;13:532. doi: 10.1038/s41467-022-28199-7

Table 3.

Linear regression analyses for the RT-qPCR Ct value across the different categories of infections, adjusting for sex, age, nationality, reason for RT-qPCR testing, and calendar week of RT-qPCR testa.

Original sample size RT-qPCR Ct value Univariable regression analysis Multivariable regression analysis
N (%) Mean (SD) p value β (95% CI) p value Adjusted β (95% CI) p value
Study group
 Primary infections in unvaccinated individuals 301,424 (98.0) 24.4 (6.3) <0.001 Reference Reference
 Reinfections in unvaccinated individuals 1695 (0.6) 28.0 (6.4) 3.61 (3.30–3.91) <0.001 3.67 (3.38–3.96) <0.001
 BNT162b2-vaccine breakthrough infections 4262 (1.4) 24.9 (6.5) 0.60 (0.40–0.79) <0.001 0.82 (0.63–1.01) <0.001
 mRNA-1273-vaccine breakthrough infections 283 (0.1) 30.2 (6.0) 5.82 (5.08–6.56) <0.001 3.29 (2.58–4.00) <0.001
Sex
 Male 220,318 (71.6) 24.6 (6.4) <0.001 Reference Reference
 Female 87,346 (28.4) 23.9 (6.2) −0.61 (−0.66 to −0.56) <0.001 −0.45 (−0.50 to −0.40) <0.001
Age group
 20–29 yearsb 67,040 (21.8) 24.7 (6.5) <0.001 Reference Reference
 <10 years 21,981 (7.1) 26.4 (5.7) 1.72 (1.63–1.82) <0.001 2.13 (2.04–2.23) <0.001
 10–19 years 23,581 (7.7) 23.9 (6.0) −0.80 (-0.90 to −0.71) <0.001 −0.29 (−0.38 to −0.20) <0.001
 30–39 years 106,493 (34.6) 24.3 (6.5) −0.39 (−0.45 to −0.33) <0.001 −0.20 (−0.26 to −0.15) <0.001
 40–49 years 58,072 (18.9) 24.1 (6.3) −0.60 (−0.67 to −0.53) <0.001 −0.28 (−0.34 to −0.21) <0.001
 50–59 years 22,299 (7.2) 23.9 (6.2) −0.78 (−0.87 to −0.68) <0.001 −0.41 (−0.50 to −0.32) <0.001
 60–69 years 6360 (2.1) 23.5 (6.1) −1.15 (−1.31 to −0.98) <0.001 −0.82 (−0.97 to −0.66) <0.001
 70+ years 1838 (0.6) 23.3 (6.0) −1.38 (−1.67 to −1.09) <0.001 −1.04 (−1.32 to −0.76) <0.001
Reason for RT-qPCR testing
 Clinical suspicion 110,948 (36.1) 23.1 (6.0) <0.001 Reference Reference
 Pre-travel 4557 (1.5) 26.3 (6.4) 3.26 (3.08–3.44) <0.001 3.64 (3.46–3.82) <0.001
 Port of entry 33,287 (10.8) 26.6 (6.7) 3.56 (3.49–3.64) <0.001 3.55 (3.46–3.63) <0.001
 Survey 48,361 (15.7) 25.6 (6.3) 2.56 (2.50–2.63) <0.001 2.76 (2.69–2.82) <0.001
 Individual request 15,489 (5.0) 24.7 (6.4) 1.66 (1.55–1.76) <0.001 2.22 (2.11–2.32) <0.001
 Contact tracing 67,159 (21.8) 25.1 (6.1) 2.04 (1.98–2.10) <0.001 1.70 (1.64–1.76) <0.001
 Healthcare routine testing 21,014 (6.8) 21.5 (6.2) −1.60 (−1.69 to −1.51) <0.001 −0.84 (−0.94 to −0.75) <0.001
 Other 6849 (2.2) 25.3 (6.4) 2.21 (2.06–2.36) <0.001 1.87 (1.71–2.02) <0.001

CI confidence interval, Ct cycle threshold, RT-qPCR real-time reverse transcription polymerase chain reaction, SD standard deviation.

aAnalyses were conducted on the full sample including all infections diagnosed using TaqPath COVID-19 Combo Kits platform. Categories for calendar week of RT-qPCR test are not presented in the table for brevity.

b20–29 years was chosen as a reference category because it is the first fully adult age group.