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. 2022 Jan 27;12:1513. doi: 10.1038/s41598-022-05405-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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HOTAIR-MRPL interactions do not occur in mitochondria. (A) Overlap of ChIRP-MS and RNA-BioID identified proteins. Proteins identified in both methods are shown. (B) Reanalysis of RNA-sequencing data of Mercer et al. Nuclear-encoded genes are shown in green. Mitochondrial-encoded genes are shown in red. HOTAIR is highlighted. Colocalization of HOTAIR (C–F), PPIB mRNA positive control (G–J), and DapB mRNA negative control (K–N) with mitochondria in MCF7 determined by staining with RNAscope (FITC) and MitoTracker. (O) Determined transcript abundances per cell (n = 7) for each of the targets based on RNAscope puncti. (P) Intersect analysis (n = 7) showing percentage of the total transcript pool with mitochondria (hexagons) or nuclei (circles). Transcripts present in unstained organelle are shown as other (squares). The scale bar depicts 7.5 µm.