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. 2022 Jan 27;12:1536. doi: 10.1038/s41598-022-05195-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Specific activity and thermostability of recombinant protein for VAE and consensus hOTC variants. (A) Specific activity (μmol citrulline/min/mg protein) distribution of recombinant protein for VAE (orange) and consensus (blue) hOTC variants. Specific activity of hOTC is indicated by a green dot and the dashed line, and the mean specific activity by the solid line. Each point represents the average of three separate trials for a unique variant. (B) Melting temperature (T_m in °C) distribution of recombinant protein for VAE (orange) and consensus (blue) hOTC variants. T_m of hOTC is indicated by a green dot and dashed line, and the mean by the solid line. Each point represents the average of three separate trials for a unique variant. (C) Scatter plot of thermal stability (∆T_m in °C) vs specific activity (fold-change) for all OTC variants relative to hOTC. Histograms of each population are plotted on the top and left axes. Orange dots represent VAE variants, and blue dots represent consensus variants. Variants show a mix of improved specific activity, thermal stability, or both. The hOTC values are indicated by the dashed line and green dot.