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. 2022 Jan 27;12:1536. doi: 10.1038/s41598-022-05195-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Performance of VAE hOTC variants in mammalian cells. (A) OTC expression in HepG2 cells transfected with OTC mRNAs. Expression was normalized relative to hOTC (black dashed line). Data points indicate the average values of three biological replicates and error bars indicate standard deviation. (B) Total OTC activity (nmol/min/mg total protein normalized to hOTC) in HepG2 cells transfected with OTC mRNAs. Data points indicate the average values of three biological and six technical replicates. Black error bars represent standard deviations. (C) OTC activity in HepG2 cells transfected with OTC mRNAs. Total activity (panel B, nmol citrulline/min/mg total protein) was normalized to the expression observed in panel A to determine specific activity of VAE OTCs. Asterisks represent significance of < 0.004 under Bonferroni-corrected t test.