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. 2022 Jan 27;13(1):87. doi: 10.1038/s41419-022-04541-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a The mRNA levels of the isoforms in each family of Glut, Hk, Pfk, Aldo, Eno, and Ldh in mouse ovaries at 4 dpp. The mRNA values of Glut3, Hk3, Pfkp, Aldoc, Eno4, and Ldhd were set as 1. (n = 3 independent experiments). Bars indicate the mean ± SD. **P < 0.01 and ***P < 0.001. b The mRNA levels of Glut4, Hk1, Pfkl, Aldoa, Eno1, Tpi, Pkm2, and Ldhb in the ovaries at 1, 4 and 7 dpp. (n = 3 independent experiments). Bars indicate the mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. 1 dpp group. c, d The protein levels of GLUT4, HK1, PFKL, and PKM2 in the ovaries at 1, 4, and 7 dpp. (n = 3 independent experiments). β-actin was used as an internal control. Bars indicate the mean ± SD. **P < 0.01 and ***P < 0.001 vs. 1 dpp group. e Immunofluorescence stain of GLUT4, HK1, PFKL, and PKM2 (green) in the ovaries at 1, 4, and 7 dpp. (n = 3 independent experiments). The cytoplasm of oocytes was stained with the oocyte-specific marker DDX4 (red), and the nuclei were counterstained by DAPI (blue). The arrowheads and the arrows show the primordial and primary follicles, respectively. Scale bars: 50 μm. f–i The intensity of GLUT4 (f), HK1 (g), PFKL (h), and PKM2 (i) fluorescent signals in granulosa cells (GC) and oocytes (OO) of primordial follicles (PF) and primary follicles (PrF). (n = 9 sections from 7 dpp ovaries. The total number of 45 follicles was scored in each group). Bars indicate the mean ± SD. **P < 0.01 and ***P < 0.001. The representative images are shown.