Fig. 4.
V-CARMA can infect CD4 T cells expressing cognate TCR via displaying peptide presented by class II MHC. (A, Top) Schematic of envelope plasmid design for MBP85–99-DR15. (A, Bottom) FACS plots and quantitation graph of pHLA-PLVs displaying empty and MBP-DR15 infection to CD4+ Jurkat cells expressing Ob1A12 TCR (n = 3 per group). (B, Left) FACS plots of pHLA-PLVs displaying empty and MBP-DR15 infection to human primary CD4 T cells expressing Ob1A12 TCR. (B, Right) Quantitation graph of the flow cytometry results on the Left. Primary CD4 T cells from two individual donors were tested. Data are presented as mean ± SEM and are representative of two independent experiments. ****P < 0.0001.