TABLE 1.
Biochemical profiles of Tsukamurella species with three commercial systems
| Identification system | Biochemical profile (no. of isolates) for the following Tsukamurella species (no. of isolates):
|
||||
|---|---|---|---|---|---|
| T. strandjordae (1) | T. paurometabola (1) | T. inchonensis (4)d | T. pulmonis (4) | T. tyrosinosolvens (4) | |
| API Corynea | |||||
| 2 days | 2550004 | 2550004 | 2150004 (2), 2550004 (1), 2551004 (1) | 2150004 | 2150004 |
| 4 days | 2553004 | 2550104 | 2150004 (2), 2550004 (1), 2551004 (1) | 2152004 (1) | 2152004 (2) |
| 7 days | 2553004 | 2550104 | 2153004 (1) | 2152004 (1) | 2152004 (2) |
| RapID CB Plusb | 0627511 | 0647511 | 0607511 (3), 0623531 (1) | 0627511 | 0607511 (2), 0627511 (1), 0627531 (1) |
| API 20C AUXc | 6063160 | 6040160 | 6167171 (2), 2127171 (1), 2067171 (1) | 6062160 (2), 6040120 (1), 6042160 (1) | 6167171 (1), 6167771 (1), 2147171 (1) |
The Rapid Coryne strip contained the following reactive ingredients in the ordered groups of three as follows: nitrate reduction, pyrazinamidase, and pyrrolidonyl arylamidase; alkaline phosphatase, β-glucuronidase, and β-galactosidase, α-glucosidase, N-acety-β-glucosaminidase, and esculin; urease, gelatin hydrolysis, and fermentation control; glucose, ribose, and xylose; mannitol, maltose, and lactose; and sucrose and glycogen (catalase was the 21st test). Reaction changes observed after 2 days were all weakly positive.
RapID CB panel tests, ordered in groups of three except for the last two tests, are as follows: glucose, sucrose, and ribose; maltose, p-nitrophenyl-α-d-glucoside, and p-nitrophenyl-β-d-glucoside; p-nitrophenyl-N-acetyl-β-d-glucosaminide, p-nitrophenyl-glycoside, and o-nitrophenyl-β-d-galactoside; p-nitrophenylphosphate, fatty acid ester, and proline-β-naphthylamide; tryptophane-β-naphthylamide, pyrrolidine-β-naphthylamine, and leucyl-glycine-β-naphthylamide; leucine-β-naphthylamide, urea, and potassium nitrate; and catalase and yellow pigment. Reactions were recorded after 4 h.
API 20 C AUX biochemicals, ordered in groups of three except for the first two and the last two tests, are as follows: glucose and glycerol; 2-keto-d-gluconate, l-arabinose, and d-xylose; adonitol, xylitol, and galactose; inositol, sorbitol, and α-methyl-d-glucoside; N-acetyl-d-glucosamine, cellobiose, and lactose; maltose, sucrose, and trehalose; and melezitose and raffinose. The first cupule is a growth control and is not scored. Reactions were recorded after 6 days.
Includes T. paurometabola ATCC 25938.