TABLE 3.

Short-chain fatty acid content of 15 strains of Tsukamurella analyzed with the MIS

Fatty acid	Mean ± SD fatty acid content (%)
	T. strandjordae		T. paurometabola		T. inchonensis		T. pulmonis		T. tyrosinosolvens		T. wratislaviensis
	Clinical method	Mycobacteriological method	Clinical method	Mycobacteriological method	Clinical method	Mycobacteriological method	Clinical method	Mycobacteriological method	Clinical method	Mycobacteriological method	Clinical method	Mycobacteriological method
C12:0	—f	—	—	—	—	—	—	—	—	0.37 ± 0.02	—	—
C14:0	4.94	5.36	3.81	5.15	4.83 ± 0.82	3.48 ± 0.93	4.05 ± 0.69	3.28 ± 0.57	3.93 ± 0.45	5.95 ± 1.34	3.57	3.52
C15:0	1.00	0.36	—	—	0.68 ± 0.21	0.5 ± 0.25	0.75 ± 0.14	0.27 ± 0.01	0.81 ± 0.24	—	3.92	2.52
C16:1ω9	—	0.86	—	1.68	0.62 ± 0.98	1.35 ± 0.55	1.48 ± 0.42	2.42 ± 0.43	—	1.01 ± 0.28	—	0.60
C16:1ω6	—	2.73	—	10.59	—	4.62 ± 1.69	—	4.91 ± 1.75	—	3.15 ± 1.26	—	8.66
C16:0	40.36	40.69	36.89	36.72	38.88 ± 2.67	37.74 ± 2.6	33.73 ± 3.54	31.08 ± 2.68	42.89 ± 1.52	39.32 ± 2.44	35.45	30.06
C17:1ω8	—	—	—	—	—	—	—	—	—	—	3.90	4.69
C17:0	1.78	0.40	1.57	—	1.04 ± 0.29	0.65 ± 0.42	1.44 ± 0.37	0.32 ± 0.01	1.36 ± 0.57	—	2.68	3.80
C17:0 10 methyl	—	—	—	—	—	—	—	—	—	—	2.01	—
C18:1ω9	23.70	17.72	26.43	13.39	24.79 ± 2.58	17.94 ± 6.76	30.86 ± 6.82	30.23 ± 12.15	29.59 ± 3.83	20.11 ± 6.23	21.47	22.28
C18:0	2.18	11.13	3.30	6.66	2.78 ± 1.31	9.41 ± 4.67	4.95 ± 1.54	8.21 ± 2.24	2.72 ± 0.77	8.67 ± 3.5	2.44	2.34
TBSAa	16.58	18.51	16.49	25.81	16.7 ± 4.37	24.28 ± 7.83	10.07 ± 8.34	17.28 ± 12.72	13.21 ± 1.52	20.63 ± 3.74	12.73	12.02
C20:1ω9	1.65	—	1.69	—	1.59 ± 0.29	—	5.03 ± 0.34	—	1.18 ± 0.24	—	—	—
C20:0	—	1.87	—	—	—	1.26 ± 0.99	0.39 ± 0.5	2.15 ± 0.99	—	1.23 ± 0.65	—	0.58
Sum 1b	—	0.37	—	—	—	0.52 ± 0.23	—	—	—	—	—	1.00
Sum 3c	7.81	—	9.20	—	7.07 ± 2.46	—	6.33 ± 2.85	—	—	—	11.83	7.28
Sum 5d	—	—	0.62	—	0.68 ± 0.31	—	0.74 ± 0.42	—	0.84 ± 0.46	—	—	—
Sum 6e	—	—	—	—	0.97 ± 0.58	—	0.75 ± 0.3	—	—	—	—	—

^aTBSA, tuberculostearic acid (C_{18:0 10 methyl}). 

^bSum 1, summed feature 1 (C_{16:0 8 methyl} or C_{16:0 10 methyl}; these fatty acids elute at the same time and therefore cannot be differentiated). 

^cSum 3, summed feature 3 (C_16:1ω7 or C_{15 iso 2OH}). 

^dSum 5, summed feature 5 (C_18:2ω6,9 or C_{18:0 Ante}). 

^eSum 6, summed feature 6 (C_19:1ω9 or C_19:1ω1). 

^f—, fatty acid not detected.