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. 2022 Jan 14;12:818334. doi: 10.3389/fgene.2021.818334

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Copyright © 2022 Kim, MacIntyre, Sykes, Arianoglou, Bennett and Terzidou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of hemolysis on plasma miRNA expression profiles. Risk of hemolysis was found to be higher with increasing blood holding time. Correlation between plasma miRNAs and hemolysis was assessed. The Cq values of four miRNAs demonstrated positive correlation with hemolysis (A) and Cq values of two miRNAs demonstrated negative correlation with hemolysis (B). Venn diagram showing the number of miRNAs whose expression is affected by whole blood holding time prior to plasma processing and/or level of hemolysis (C).