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. 2022 Jan 14;11:808550. doi: 10.3389/fcimb.2021.808550

Figure 4.

Figure 4

pH of the culture medium impacts the ability of F. tularensis to form biofilm. (A) LVS biofilm formation was assessed in Chamberlain’s defined medium (CDM), brain-heart infusion broth (BHI) supplemented with 1% IsoVitaleX and modified Mueller-Hinton broth (MMH) supplemented with 2% IsoVitaleX. Biofilms were assayed at 10 days post-inoculation. (B) LVS grown in either CDM or BHI for 7 days was sub-cultured via replica plating into CDM or BHI. (C) LVS biofilm formation was assessed after culturing for 7 days in CDM or BHI pH adjusted to 6.2 or 7. (D) The effect of culture medium pH was assessed in fully virulent Type A and B isolates (FRAN 244 and FRAN255, respectively). The red bar indicates the median value after at least 3 independent experiments. **P < 0.01.