Figure 5.

pH acts as an environmental checkpoint for initiation of biofilm matrix assembly. LVS biofilms were grown in CDM adjusted to the indicated pH. After 7 days, bacteria were sub-cultured into either CDM at pH 6.2 or 7 and the progeny biofilms were stained at 24 h. (A) Representative images of biofilm plates crossed into CDM at each pH. (B) Graphed data after 3 independent experiments. Red bars indicates the median. OG indicates the original plates from which progeny plates were inoculated.