Figure 3.

Effects of the NLRP3 inhibitor MCC950 and colchicine on cytokine secretion by COVID-19 PBMCs. IL-1β (A), IL-6 (B) and TNFα (C) levels from supernatants of healthy control (HC, n = 7) or COVID-19 patients PBMCs (n = 16) after a 4-hour incubation at 37°C, were determined by multi-analyte flow assay kit. Lines represent median values and interquartile ranges. Data were analyzed using the Mann–Whitney test. ***P < 0.001, ****P < 0.0001. Alternatively, COVID-19 cells were treated with the NLRP3 inhibitor, MCC950 (10 μM; n = 9) (D) or colchicine (10 μM; n = 7) (E) during the incubation period, and cytokine levels were measured in the culture supernatants. Numbers represent the median values and interquartile ranges with or without treatment. Paired analysis was performed using the Wilcoxon test. *P < 0.05; **P < 0.01; ns, not significant.