Skip to main content
. 2022 Jan 28;8:38. doi: 10.1038/s41420-022-00831-x

Fig. 3. Single-cell transcriptional analysis reveals heterogeneity of alveolar epithelial cells in FHP patients.

Fig. 3

A t-SNE visualization of AT1 cells, AT2 cells, club cells, ciliated cells, and KRT17high basal-like cells. AT1: alveolar type I cells. AT2: alveolar type II cells. B The expression levels of top five marker genes in cell subtypes. C Relative percentage of sample origins across cell subtypes. D, E AUCell analyses of the relative gene set enrichment scores in Lung Basal Cell signature (D) and EMT signature (E). F Multiplex immunofluorescence images of AGER, SFTPC, and KRT17 in lung samples from the patient with FHP. Scale bar = 10 μm. G Trajectory analysis of AT1 cells, AT2 cells, club cells, and KRT17high basal-like cells using Monocle 2 (upper) and Velocyto R package (lower). H SOX4_extended motif showed greater enrichment of regulon activity for KRT17high basal-like cells using SCENIC analysis. I t-SNE visualization of AUC values of SOX4_extended motif in alveolar epithelial cells. J t-SNE visualization of expression levels of SOX4 in alveolar epithelial cells. K Schematic developmental trajectories of KRT17high basal-like cell in FHP lungs. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.