REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Sheep polyclonal anti-NPHS1	R&D Systems	Cat. #AF4269; RRID:AB_2154851
Rabbit monoclonal anti-PDGFRβ (clone C82A3)	Cell Signaling Technology	Cat. #4564; RRID:AB_2236927
Mouse monoclonal anti-E-Cadherin (clone 36)	BD Transduction laboratories	Cat. #610181; RRID:AB_397580
Goat polyclonal anti-ACE2	R&D Systems	Cat. #AF933; RRID:AB_355722
Rabbit polyclonal anti-ACE2	ProteinTech	Cat. #21115-1-AP; RRID:AB_10732845
Rabbit monoclonal anti-ACE1 (clone EPR22291-247)	Abcam	Cat. #254222
Mouse monoclonal anti-LRP2 (clone H-10)	Santa Cruz Biotechnology	Cat. #sc-515772; RRID:AB_2783023
Rabbit monoclonal anti-GAPDH (clone 14C10)	Cell Signaling Technology	Cat. #2118; RRID:AB_561053
Alexa Fluor 405 conjugated anti-mouse IgG	Abcam	Cat. # ab175658; RRID:AB_2687445
Alexa Fluor 488 conjugated anti-mouse IgG	Thermo Fisher Scientific	Cat. #A-21202; RRID:AB_141607
Alexa Fluor 555 conjugated anti-goat IgG	Thermo Fisher Scientific	Cat. #A-21432; RRID: AB_2535853
Alexa Fluor 568 conjugated anti-rabbit IgG	Thermo Fisher Scientific	Cat. #A-10042; RRID:AB_2757564
Alexa Fluor 568 conjugated anti-sheep IgG	Thermo Fisher Scientific	Cat. #A-21099; RRID:AB_2535753
Alexa Fluor 647 conjugated anti-mouse IgG	Thermo Fisher Scientific	Cat. #A-31571; RRID:AB_162542
Alexa Fluor 647 conjugated anti-sheep IgG	Thermo Fisher Scientific	Cat. #A-21448; RRID:AB_1500712
Lotus Tetragonolobus Lectin (LTL), Fluorescein labelled	Vector Laboratories	Cat. #FL-1321-2; RRID:AB_2336559
Lotus Tetragonolobus Lectin (LTL), Cy5	GlycoMatrix	Cat. #21761117-1
Biological samples
Human kidney tissue	Non-diseased segments of human kidney nephrectomy samples obtained from deidentified patients approved by the Conjoint Health Research Ethics Board (CHREB) at the University of Calgary	n/a
Chemicals, peptides, and recombinant proteins
mTeSR™1	Stem Cell Technologies	Cat. #85850
Gentle Cell Dissociation Reagent	Stem Cell Technologies	Cat. #7174
STEMdiff™ APEL2™ medium	Stem Cell Technologies	Cat. #05275
Protein-Free Hybridoma Medium	Thermo Fisher Scientific	Cat. #12040077
0.05% Trypsin-EDTA	Thermo Fisher Scientific	Cat. #25300-062
Dulbecco's modified Eagle's medium	Thermo Fisher Scientific	Cat. #11965-092
Dulbecco's modified Eagle's medium/F12	Thermo Fisher Scientific	Cat. #11330032
Advanced RPMI 1640 medium	Thermo Fisher Scientific	Cat. #12633012
Embryonic stem cell-qualified fetal bovine serum	Thermo Fisher Scientific	Cat. #10439016
Matrigel hESC-Qualified Matrix LDEV-free	Corning	Cat. #354277
Donkey serum	Millipore Sigma	Cat. #S30
Fetal Bovine Serum	Millipore Sigma	Cat. #F1051
Angiotensin II	Millipore Sigma	Cat. #A9525
Heparin sodium salt	Millipore Sigma	Cat. #4784
CHIR99021	R&D Systems	Cat. #4423
InSolution™ Y-27632	Millipore Sigma	Cat. #688001
16% aqueous Paraformaldehyde (PFA)	EMS	Cat. #15710
Recombinant human FGF-9	R&D Systems	Cat. #273-F9-025
Losartan	Tocris	Cat. #3798
Hoechst 33342	Thermo Fisher Scientific	Cat. #H3570
Penicillin/streptomycin (100x)	Thermo Fisher Scientific	Cat. #15070-063
Penicillin-Streptomycin-Glutamine (100X)	Thermo Fisher Scientific	Cat. #10378016
Collagenase IV	Worthington	Cat. #LS004188
Prostaglandin E1	Millipore Sigma	Cat. #P8908
Human EGF	Millipore Sigma	Cat. #SRP3027
T3 (3,3’,5-triiodo-L-thyronine)	Millipore Sigma	Cat. #T2877
Hydrocortisone	Millipore Sigma	Cat. #H4001
ITSS	Millipore Sigma	Cat. #I1884-1VL
TrypLE Select	Thermo Fisher Scientific	Cat. #12563011
Critical commercial assays
10X Chromium™ Controller	10X Genomics	N/A
Chromium™ Single Cell A Chip kit, 48 rxns	10X Genomics	Cat. #120236
Chromium™ Single cell 3′Library & Gel beaded kit V3 and 3.1 Next GEM, 16 rxns	10X Genomics	Cat. #1000075; Cat. #1000128
Chromium i7 multiplex Kit 96 rxns	10X Genomics	Cat. #120262
Illumina NovaSeq S2 Flowcell	Illumina, Centre for Health Genomics and Informatics, University of Calgary	N/A
Scepter™ 2.0 Handheld Automated Cell Counter	Millipore	Cat. #PHCC20040
Agilent D1000 ScreenTape System	Agilent Technologies	Part # 5067-5582
Deposited data
Kidney organoid scRNA-seq	This study	GEO:GSE149687
SARS-CoV-2 infectable kidney organoids scRNA-seq	Monteil et al. (2020)	GEO:GSNE147863
Experimental models: cell lines
Human iPSC – Gibco episomal iPSC line	Thermo Fisher Scientific	Cat. #A18945
Human iPSC line (derived from human dermal fibroblast, Cell Applications, Inc. Cat#106-05n, Lot# 1481)	Gift from Dr. Martin Pollak, Harvard Medical School	n/a
Vero E6	ATCC	Cat. #C1008
Software and algorithms
ImageJ	National Institutes of Health	https://imagej.nih.gov/ij/; RRID: SCR_003070
GraphPad Prism 8.3.0	GraphPad	https://www.graphpad.com/;SCR_015807
R Studio	https://www.rstudio.com/products/rstudio/	Version 3.6.2-4.1.0
R	https://www.r-project.org/	Version 3.6.2-4.0.5
Cell Ranger	10X Genomics	Version 3.0.1
Seurat	https://github.com/satijalab/seurat	Version 3.2.3 - 4.0.3
Leica software	Leica	N/A
BioRender	BioRender.com
Other
μ-slide 8 well glass-bottom chamber	ibidi	Cat. #80826
Transwell	Corning	Cat. #07-200-170
Human Protein Atlas	https://www.proteinatlas.org/	Version 19.3