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EerI administration represses UUO-induced Klotho protein loss in mice. (a) Representative immunohistochemical staining image of Klotho expression (scale bar, 100 μm). (b) PCR analysis of Klotho in renal tissue and their relative expression levels. (c) Western blotting analysis of Klotho in renal tissue and their relative expression levels. (d)Serum sKlotho levels in mice detected by ELISA. Data are presented as the mean ± SD (n = 6).
