TABLE 2.
Differentially expressed genes (mRNA) between embryos produced from low fertility (LF) and high fertility (HF) bulls. L = mean normalized count values in LF embryos. H = mean normalized count values in HF embryos.
| ENSEMBL ID | Gene symbol | L | H | Log2 fold change | Adj.p-value | Functional description |
|---|---|---|---|---|---|---|
| Highly expressed in LF embryos | ||||||
| ENSBTAG00000014046 | BPI | 573 | 0 | 10.90 | 1.72E-03 | Bactericidal permeability increasing protein |
| ENSBTAG00000003305 | NCF1 | 16 | 0 | 5.81 | 6.69E-03 | Neutrophil cytosol factor 1 |
| ENSBTAG00000047563 | CLDN9 | 142 | 11 | 3.69 | 6.76E-03 | Claudin 9 |
| ENSBTAG00000026893 | EXOC3L4 | 496 | 52 | 3.26 | 1.70E-03 | Bos taurus exocyst complex component 3 like 4 |
| ENSBTAG00000049434 | Non-annotated gene | 238 | 30 | 2.99 | 2.52E-03 | |
| ENSBTAG00000051376 | Non-annotated gene | 33 | 6 | 2.43 | 2.52E-03 | |
| ENSBTAG00000013854 | CALML5 | 963 | 213 | 2.17 | 7.14E-10 | Calmodulin like 5 |
| ENSBTAG00000011839 | HMGCS1 | 1949 | 471 | 2.05 | 6.13E-04 | Bos taurus 3-hydroxy-3-methylglutaryl-CoA synthase 1 |
| ENSBTAG00000054516 | CYP17A1 | 293 | 76 | 1.95 | 2.60E-02 | Steroid 17-alpha-hydroxylase/17,20 lyase |
| ENSBTAG00000017819 | PMVK | 310 | 93 | 1.74 | 1.21E-02 | Phosphomevalonate kinase |
| ENSBTAG00000004905 | KRT19 | 24,981 | 7,880 | 1.67 | 2.83E-02 | Keratin 19 |
| ENSBTAG00000003068 | MSMO1 | 3,085 | 1,031 | 1.58 | 6.49E-04 | Methylsterol monooxygenase 1 |
| ENSBTAG00000006305 | AK1 | 184 | 65 | 1.51 | 4.30E-03 | Bos taurus adenylate kinase 1 |
| ENSBTAG00000017864 | PRPH | 904 | 326 | 1.47 | 2.52E-03 | Bos taurus peripherin |
| ENSBTAG00000055207 | SCD | 1,324 | 478 | 1.47 | 4.39E-03 | Bos taurus stearoyl-CoA desaturase |
| ENSBTAG00000004075 | IDI1 | 1,616 | 618 | 1.39 | 2.60E-04 | Bos taurus isopentenyl-diphosphate delta isomerase 1 |
| ENSBTAG00000012432 | FDFT1 | 2,967 | 1,145 | 1.37 | 1.05E-02 | Bos taurus farnesyl-diphosphate farnesyltransferase 1 |
| ENSBTAG00000007840 | HMGCR | 1,178 | 465 | 1.34 | 4.30E-03 | Bos taurus 3-hydroxy-3-methylglutaryl-CoA reductase |
| ENSBTAG00000004881 | MTHFD2 | 1,085 | 440 | 1.30 | 2.84E-03 | Bos taurus methylenetetrahydrofolate dehydrogenase (NADP + dependent) 2, methenyltetrahydrofolate cyclohydrolase |
| ENSBTAG00000014127 | PTGS2 | 7,075 | 2,949 | 1.26 | 4.30E-03 | Bos taurus prostaglandin-endoperoxide synthase 2 |
| ENSBTAG00000055124 | Non-annotated gene | 53 | 23 | 1.23 | 2.76E-03 | |
| ENSBTAG00000003948 | FDPS | 1,516 | 708 | 1.10 | 2.52E-03 | Farnesyl diphosphate synthase |
| ENSBTAG00000003100 | SMTN | 209 | 100 | 1.05 | 2.76E-02 | Bos taurus smoothelin |
| ENSBTAG00000004982 | GPLD1 | 969 | 470 | 1.04 | 1.53E-02 | Bos taurus glycosylphosphatidylinositol specific phospholipase D1 |
| ENSBTAG00000032914 | SLC11A2 | 1,023 | 513 | 1.00 | 2.51E-02 | Bos taurus solute carrier family 11 member 2 |
| ENSBTAG00000006471 | OSBPL11 | 198 | 102 | 0.96 | 2.75E-02 | Oxysterol binding protein like 11 |
| ENSBTAG00000014227 | NDFIP2 | 2,381 | 1,229 | 0.95 | 4.30E-03 | Bos taurus Nedd4 family interacting protein 2 |
| ENSBTAG00000019246 | SC5D | 605 | 319 | 0.92 | 4.56E-02 | Bos taurus sterol-C5-desaturase |
| ENSBTAG00000055014 | SH3BGRL2 | 2,429 | 1,352 | 0.85 | 3.07E-02 | Bos taurus SH3 domain binding glutamate rich protein like 2 |
| ENSBTAG00000044015 | RBM12 | 285 | 172 | 0.73 | 2.73E-02 | Bos taurus RNA binding motif protein 12 |
| ENSBTAG00000012317 | PNP | 3,762 | 2,302 | 0.71 | 4.78E-02 | Bos taurus purine nucleoside phosphorylase |
| ENSBTAG00000016896 | HERPUD1 | 531 | 327 | 0.70 | 2.52E-03 | Homocysteine inducible ER protein with ubiquitin like domain 1 |
| ENSBTAG00000017258 | ACSL3 | 6,014 | 3,780 | 0.67 | 2.70E-02 | Bos taurus acyl-CoA synthetase long chain family member 3 |
| ENSBTAG00000011899 | USP4 | 1,339 | 858 | 0.64 | 6.95E-03 | Ubiquitin specific peptidase 4 |
| Highly expressed in HF embryos | ||||||
| ENSBTAG00000031825 | Non-annotated gene | 0 | 45 | 8.02 | 2.60E-04 | |
| ENSBTAG00000046257 | GIMAP4 | 1 | 68 | 5.85 | 3.57E-02 | Bos taurus GTPase, IMAP family member 4 |
| ENSBTAG00000014560 | HLX | 0 | 9 | 5.72 | 3.80E-02 | Bos taurus H2.0 like homeobox |
| ENSBTAG00000030882 | hsd20b2 | 7 | 81 | 3.48 | 1.85E-02 | Bos taurus estradiol 17-beta-dehydrogenase 12-like (LOC508455) |
| ENSBTAG00000015836 | Non-annotated gene | 50 | 351 | 2.82 | 1.91E-04 | |
| ENSBTAG00000010123 | APOE | 22 | 135 | 2.65 | 2.36E-02 | Apolipoprotein E |
| ENSBTAG00000014596 | EFHD1 | 153 | 823 | 2.43 | 1.70E-03 | EF-hand domain family member D1 |
| ENSBTAG00000027444 | SVIL | 12 | 60 | 2.37 | 4.98E-02 | Bos taurus supervillin |
| ENSBTAG00000054434 | Non-annotated gene | 6 | 25 | 2.08 | 3.33E-02 | |
| ENSBTAG00000033429 | FAM229B | 7 | 26 | 1.96 | 4.92E-02 | Bos taurus family with sequence similarity 229 member B |
| ENSBTAG00000049950 | Non-annotated gene | 9 | 32 | 1.86 | 4.92E-02 | |
| ENSBTAG00000026758 | Non-annotated gene | 22 | 77 | 1.83 | 2.60E-02 | |
| ENSBTAG00000017094 | SHMT1 | 63 | 209 | 1.72 | 2.73E-02 | Serine hydroxymethyltransferase 1 |
| ENSBTAG00000038384 | KRT5 | 434 | 1,210 | 1.48 | 1.21E-02 | Keratin 5 |
| ENSBTAG00000054234 | Non-annotated gene | 228 | 632 | 1.47 | 2.86E-02 | |
| ENSBTAG00000003568 | CLDN10 | 417 | 1,120 | 1.43 | 4.44E-03 | Bos taurus claudin 10, transcript variant 2 |
| ENSBTAG00000004386 | SOCS1 | 105 | 274 | 1.39 | 4.30E-03 | Suppressor of cytokine signaling 1 |
| ENSBTAG00000012511 | BAD | 306 | 787 | 1.36 | 1.72E-03 | Bos taurus BCL2 associated agonist of cell death |
| ENSBTAG00000003043 | GNG2 | 148 | 373 | 1.33 | 2.09E-02 | G protein subunit gamma 2 |
| ENSBTAG00000006086 | MMP28 | 110 | 278 | 1.33 | 1.72E-03 | Matrix metallopeptidase 28 |
| ENSBTAG00000022028 | DERL3 | 114 | 257 | 1.17 | 2.52E-03 | Bos taurus derlin 3 |
| ENSBTAG00000013922 | MOSPD1 | 196 | 435 | 1.15 | 4.47E-02 | Bos taurus motile sperm domain containing 1 |
| ENSBTAG00000020528 | PCOLCE | 126 | 273 | 1.12 | 1.01E-02 | Bos taurus procollagen C-endopeptidase enhancer |
| ENSBTAG00000003222 | ASNS | 287 | 574 | 1.00 | 4.18E-02 | Asparagine synthetase (glutamine-hydrolyzing) |
| ENSBTAG00000010740 | CLTB | 2,843 | 5,223 | 0.88 | 4.21E-02 | Clathrin light chain B |
| ENSBTAG00000052249 | Non-annotated gene | 41 | 71 | 0.80 | 2.60E-02 | |
| ENSBTAG00000021111 | POU5F1 | 10,804 | 18,443 | 0.77 | 2.26E-03 | Bos taurus POU class 5 homeobox 1 |
| ENSBTAG00000017932 | CCDC84 | 183 | 286 | 0.65 | 3.75E-02 | Bos taurus coiled-coil domain containing 84 |