For organ harvest, mice were deeply anesthetized with 5% isoflurane, sacrificed by cervical dislocation and organs were harvested in the following order.
Organ | Histology | Homogenization | Flow cytometry |
---|---|---|---|
spleen | 50% | 50% | – |
kidney | one kidney | one kidney | – |
liver | ca. 5 × 10 mm piece | ca. 5 × 10 mm piece | – |
lung | Right lobe | Left lung and one small right lobe | rest |
heart | 50% | 50% | – |
Submandibular salivary gland | one lobe | one lobe | – |
trachea | upper 3mm | Rest | – |
brain | one lobe | one lobe | – |
ileum | ca. 5 cm | ca. 5 cm | – |
feces | – | taken directly from colon if applicable | – |
colon | ca. 3 cm | ca. 3 cm |