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. 2022 Jan 14;9:787278. doi: 10.3389/fcell.2021.787278

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Copyright © 2022 Xie, Shi, Chen, Zhou, Luo, Hong, Tian, Wu, Zhou, Lin, Dou, Wu, Huang, Zhang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of apigenin (API) on cell viability, apoptosis, and senescence of rat nucleus pulposus (NP) cells. (A) Chemical structure of API. (B,C) Counting Kit-8 (CCK8) results of NP cells treated with various concentrations of API or TBHP for 24 h. (D) CCK-8 results of NP cells co-treated with API and TBHP (50 μM) for 24 h. (E,F) Live and dead staining of NP cells cocultured with API and TBHP (50 μM) for 24 h. (scale bar: 50 μm). (G) Western blot results of Bax, cleaved caspase 3, Bcl2, p21, and p16INK4a expression levels in NP cells. (H) Quantification of Bax, cleaved caspase 3, Bcl2, p21, and p16INK4a by immunoblot. (I) TUNEL and SA-β-gel staining was performed in NP cells (scale bar: 50 μm). (J) The positive cells in TUNEL and SA-β-gel staining were quantified. The data in the figures represent mean ± S.D. Significant differences between groups are indicated as ****p < 0.0001, ***p < 0.001.