TABLE 3.
X-ray and ss-NMR structures of the complexes formed by the wild type M2 proton channel and mutated variants with Amt and Rmt.
| PDB ID | Construct (mutant) | Method | Experimental conditions | References |
|---|---|---|---|---|
Amantadine
| ||||
| 2KAD | 22–46 (L40A) | ss-NMR | pH: 7.5 T: 243 K | Cady et al. (2009) |
| DLPC bilayer a | ||||
| 3C9J | 22–46 (G34A) | X-ray (3.50 Å) | pH 5.3 T: 298/100 K b | Stouffer et al. (2008) |
| OG c | ||||
| 2KQT | 22–46 | ss-NMR | pH 7.5 T: 243 K | Cady et al. (2010) |
| DMPC bilayer d | ||||
| 6BKK | 22–46 | X-ray (2.00 Å) | pH 5.6 T: 293/100 K | Thomaston et al. (2018) |
| LCP (MNG-3-C8) e | ||||
Rimantadine
| ||||
| 2RLF (R-Rmt) | 18–60 | NMR | pH: 7.5 T: 303 K | Schnell and Chou, (2008) |
| DHPC f | ||||
| 6BKL (R,S-Rmt) | 22–46 | X-ray (2.00 Å) | pH 4.5 T: 293/100 K | Thomaston et al. (2018) |
| LCP (MNG-3-C8) | ||||
| 6BOC | 22–46 | X-ray (2.25 Å) | pH 3.5 T: 293/100 K | Thomaston et al. (2018) |
| LCP (MNG-3-C8) | ||||
| 6US8 (S-Rmt) | 22–46 | X-ray (1.70 Å) | pH: 7.5 T: 293/100 K | Thomaston et al. (2021) |
| LCP (MNG-3-C8) | ||||
| 6US9 (R-Rmt) | 22–46 | X-ray (2.00 Å) | pH: 8.5 T: 293/100 K | Thomaston et al. (2021) |
| LCP (MNG-3-C8) | ||||
a1,2-Dilauroyl-sn-glycero-3-phosphatidylcholine.
t
bTemperature of crystallization/temperature of data collection in Kelvin.
cOctyl-β-D-glucopyranoside.
y
dDimyristoylphosphatidylcholine.
e
Structures obtained consideringa lipidic cubic phase (LPC) formed by monoolein and maltose neopentyl glycol analogue (MNG-3-C8).
f
Dihexanoylphosphocholine.