TABLE 2.
Comparison of the reactions of ligands and anti-GM1 antibodies to pure and miniphenol-water-extracted LPSs for validation purposes
| C. jejuni strain designation (serotype) | Strength of reaction with LPSa:
|
|||||
|---|---|---|---|---|---|---|
| CT
|
PNA
|
Anti-GM1 antibodies
|
||||
| Pure LPS | Mini- LPSd | Pure LPS | Mini- LPSb | Pure LPS | Mini- LPS | |
| NCTC 11168 (O:2) | ++ | +++ | +++ | ++ | + | ++ |
| ATCC 43441 (O:3) | − | − | − | − | − | − |
| ATCC 43442 (O:4) | ++ | + | − | − | − | − |
| ATCC 43446 (O:19) | ++++ | ++ | + | + | ++ | + |
| AZR6491 (O:23) | +++ | +++ | − | − | ++ | ++ |
| 16971.94GSH (O:41) | ++++ | ++++ | ++ | + | ++ | ++ |
| 260.94RXH (O:41) | ++++ | +++ | ++ | + | ++ | ++ |
| 28134.94GSH (O:41) | +++ | ++ | +++ | + | ++ | ++ |
| 176.83 (O:41) | ++++ | +++ | ++ | + | ++ | +++ |
a
++++, very strong reaction; +++, strong reaction; ++, moderate reaction; +, weak reaction; (+), barely visible reaction; −, no reaction.
b
Mini-LPS, LPS extracted by the miniphenol-water extraction procedure.