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. 2022 Jan 14;12:799130. doi: 10.3389/fphar.2021.799130

FIGURE 4.

FIGURE 4

The effect of PDGF-BB on the anabolism and catabolism of ECM in NPCs treated with IL-1β. (A,B) Toluidine blue staining of NP cells on high-density culture after treatment with IL-1β, PDGF-BB and Imatinib for 7 days. Scale bar, 5 mm. (C) Representative images of NP cells treated with IL-1β and PDGF-BB for 48 h. Scale bar, 100 μm. (D) The expression of anabolic-related proteins and catabolism-related proteins were measured by western blot. All NP cells were treated with or without IL-1β (10 ng/ml) and PDGF-BB for 48 h. (E) The expression of anabolic-related proteins and catabolism-related proteins were measured by western blot. All NP cells were treated with or without IL-1β (10 ng/ml) and Imatinib for 48 h. (F) The mRNA expression levels of Aggrecan, COL2, MMP3, MMP9, Adamts4 and Adamts5 were measured by qPCR. The expression of these genes was normalized to the expression of GAPDH. Data are presented as the mean ± SD. n = 3. Significant differences between groups are indicated as ***p < 0.001, **p < 0.01.