DNA oligonucleotides (all sequences are 5′ to 3′)
Cloning
Constructs	Primer pairs (fragments)
ECT2pro:ECT2-FLAG-ADAR-ECT2ter (in pCAMBIA3300-U)	LA336-695 (ECT2pro:ECT2), LA696-615 (FLAG-ADAR),LA616-337 (ECT2ter)
ECT2pro:FLAG-ADAR-ECT2ter(in pCAMBIA3300-U)	LA336-697 (ECT2pro), LA698-615 (FLAG-ADAR), LA616-337 (ECT2ter)
AGO1pro:FLAG-ADAR-AGO1ter(in pCAMBIA3300-U)	MVUSER1-11 (AGO1pro-FLAG), MVUSER12-22 (ADAR),MVUSER23-6 (AGO1ter)
FLAG-ADAR (in pGEM-T Easy)	LA696-615 (FLAG-ADAR)
Primers for USER cloning
LA336.U-ECT2P.F	GGCTTAAUAAGCAACGAACCAAGGGAAGACG
LA337.ECT2T-U.R	GGTTTAAUAGGTTCTCTCGGCTTCTTTGAC
LA615.dADAR/ECT2T.R	AGTTATUCGGCAAGACCGAACTCGTC
LA616.dADAR/ECT2T.F	AATAACUAAGAGGATGGTGTCGCTC
LA695.ECT2/FLAG.R	ATCGCAACCAUTTGCCACCACATCG
LA696.ECT2/FLAG.F	ATGGTTGCGAUTACAAGGATGACGATGAC
LA697.ECT2P/FLAG.R	AATCCAUGAGAGGAGATTCGACAAACAAAG
LA698.ECT2P/FLAG.F	ATGGATUACAAGGATGACGATGAC
MVUSER1.F	GGCTTAAUCTATCCAAATTCCAAACCATACG
MVUSER6.R	GGTTTAAUGATTCTGTCGATTGCTTTGCTGG
MVUSER11.R	ATTGGACTGUACTTGTCATCGTCATCCTTG
MVUSER12.F	ACAGTCCAAUGGTGGTGCCACAG
MVUSER22.R	ACTGCGGCAGCUCATTCGGCAAGACCGAACTCG
MVUSER23.F	AGCTGCCGCAGUTGATTCACCCTCTATCTATCTTTATGACC
Primers for site-directed mutagenesis (QuickChange)
LA729.dADAR_E488Q_QC.F	CAAAATCGAGTCCGGTCAGGGGACGATTCCAG
LA730.dADAR_E488Q_QC.R	CTGGAATCGTCCCCTGACCGGACTCGATTTTG
Primers for detection of point mutations
LA660.dADAR_E488Q_CP(NlaIII).F	CGAAAACGACACTGGTGTTG
LA735.dADAR_E488Q_CP(NlaIII).R	GCTTTTCACTGGAATCGTCCCAT