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. Author manuscript; available in PMC: 2022 Jan 28.
Published in final edited form as: Biochim Biophys Acta. 2012 Jun 9;1818(11):2521–2528. doi: 10.1016/j.bbamem.2012.06.002

Fig. 5.

Fig. 5.

Inhibition of MPERp-induced vesicle contents leakage by 4E10. A) Effect of antibody addition to the ongoing leakage. POPC:Chol (2:1, molar ratio) vesicle samples (100 μM lipid) were treated with 1 μM peptide and, subsequently supplemented with 10 μg/ml of 4E10 (addition time indicated by the arrow). The dotted traces follow the leakage kinetics in the absence of antibody. B) 4E10-induced inhibition percentages plotted as a function of the Chol mole fraction. Rate reduction caused by antibody with respect to the leakage control without antibody was calculated by correcting 0% extent of leakage to the time point of antibody addition, and subsequently measuring increment of leakage after 20 s in both samples. C) Peptide mass percentage distribution between the monolayer slabs under the experimental conditions used for X-ray scattering assays (indicated by the arrows in the previous panel). Preferential location of the peptide into the HG slab correlates with better 4E10 epitope recognition-blocking.