(A) Schematic illustration of our screening of various signaling
probe candidates on HEP-based LF strip and (B) corresponding screening
result with 30 and 5 min incubation. (C) Alignment of hep40mer and
NTD Ab to the spike. (D) Computational model of NTD and RBD epitopes
(4A8 and REGN10933, respectively) along with the ACE2 binding motif.
(E) Accessible surface area calculated from the RBD epitope (REGN10933),
ACE2 binding motif, and NTD epitope (4A8). Dark blue bars indicate
the size of the interface area as seen in Cryo-EM structures for the
RBD, ACE2, and NTD binding footprints (6XDG, 6M17, and 7C2L, respectively). p values < 0.05 (*), 0.01 (**), and 0.001 (***) determined
using a two-way ANOVA with Tukey’s post hoc test.