Table 2.

Proteins differentially regulated in vocal folds in response to acute exposure to furosemide-induced systemic dehydration.

Regulation	Molecular Pathway§	Protein IDs*	Gene symbol*	Protein Name	p-value**	Log2 FC
Downregulated	mitochondrial translation	G1U3G6	PTRH2c	Peptidyl-tRNA hydrolase 2	0.0001	−2.702
		G1SI29	TUFM	Elongation factor Tu, mitochondrial	0.0103	−0.104
		G1T752	MCTS1; LOC100352615	Malignant T-cell-amplified sequence	0.0247	−0.423
		A0A5F9CFH0	FARSB	Phenylalanyl-tRNA synthetase beta subunit	0.0289	−0.203
		G1TB33	MRPL13	Mitochondrial ribosomal protein L13	0.0308	−1.326
		G1SE37	MRPL58; ICT1	Mitochondrial ribosomal protein L58	0.0345	−2.035
		G1SZN2	MRPL38	Mitochondrial ribosomal protein L38	0.0465	−0.386
	oxidative stress	G1SND1	FMO2	Flavin Containing Dimethylaniline Monooxygenase 2	0.0035	−0.591
		G1TEA3	CAVIN2; SDPR	Caveolae associated protein 2	0.0282	−0.457
		G1SLY5	HSPB8c	Heat shock protein beta-8	0.0404	−0.700
	endocytosis	A0A5F9DCU4	DTNAc	Dystrobrevin alpha	0.0039	−1.826
		G1TKS5	OCIAD2	OCIA domain containing 2	0.0309	−0.601
		B7NZM8	YWHAH	Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta	0.0455	−0.482
	dephosphorylation	G1SS49	HDHD2c	Haloacid dehalogenase like hydrolase domain containing 2	0.0031	−3.248
	proteasomal protein catabolic process	A0A5F9C5T7	SKP1; LOC100348953	S-phase kinase-associated protein 1	0.0057	−0.776
	immune response	B5L650	RLA-A1c	MHC class I antigen	0.0134	−2.094
	RNA processing	G1SVQ4	PIN4; LOC100351985	Peptidyl-prolyl cis-trans isomerase	0.0482	−0.472
	synaptic vesicle transport	G1SKY8	TMEM230c	Transmembrane protein 230	0.0491	−1.219
Upregulated	stress response	A0A5F9CEF1	PCCA	Propionyl-CoA carboxylase subunit alpha	0.0113	0.149
		A0A0A0MQQ2	S100A12; LOC100008703	Protein S100	0.0399	4.273
	macroautophagy	G1T2T9	RAB5Af	RAB5A, member RAS oncogene family	0.0030	1.341
		G1U3Q0	ATP6V0A1	V-type proton ATPase subunit a	0.0204	0.404
	muscle tissue development	G1U3C5	IGFBP5	Insulin like growth factor binding protein 5	0.0361	0.426
		A0A5F9CCB2	KLHL40	Kelch like family member 40	0.0381	0.336
		A0A5F9DDG7	ALDH1A2f	Aldehyde dehydrogenase 1 family member A2	0.0417	1.103
		G1TKN4	RAC1	Rac family small GTPase 1	0.0434	0.253
	protein folding and sorting	A0A5F9DCR4	CALUf	Calumenin	0.0010	3.223
	N/A	A0A5F9DEL1f	N/A	Ig-like domain-containing protein	0.0115	3.368
	translation	G1SUA2	TMA7	Translation machinery associated 7 homolog	0.0226	0.308
	response to salt stress	G1TR45	EFHD2	EF-hand domain family member D2	0.0321	1.257
	acute inflammatory response	G1T127	KLKB1f	Kallikrein B1	0.0366	1.661
	energy metabolism	A0A5F9CQS8	D2HGDHf	D-2-hydroxyglutarate dehydrogenase	0.0495	1.244

^§Molecular pathways based on the most enriched GO biological process (GO-BP) or individual GO-BP annotation (in the absence of enriched term) for each protein, provided by Metascape analysis. N/A: no BP annotation provided.

^*Protein IDs and gene symbols were retrieved from Uniprot database (https://www.uniprot.org/)

^cProteins identified in the control-euhydrated group only.

^fProteins identified in the furosemide-dehydrated group only.

^**P-values from Student’s t-test. The bolded Log2 Fold Change (≥ |0.38|) proteins are considered of significant magnitude.