Table 1.
Overview of the primary antibodies used in the present study
| Antibody | Fluorophore | Clone | Species | Dilution | Source | Product number | |
|---|---|---|---|---|---|---|---|
| Tissue | In vitro | ||||||
| IHQ | FC | ||||||
| CD45 | BV425 | 30-F11 | Rat monoclonal | 1:200 | BioLegend | 103134 | |
| CD3ɛ | AF700 | 500-A2 | Armenian Hamster monoclonal | 1:100 | BioLegend | 100320 | |
| CD11b | BV510 | M1/70 | Rat monoclonal | 1:500 | BioLegend | 101263 | |
| MHC-II | PerCP-710 | AF6-120.1 | Mouse monoclonal | 1:200 | eBioscience | 46-5320-80 | |
| TLR4 | PE-Cy7 | SA15-21 | Rat monoclonal | 1:250 | BioLegend | 145407 | |
| Ly6C | BV785 | HK1.4 | Rat monoclonal | 1:2000 | BioLegend | 128041 | |
| Ly6G | AF700 | 1A8 | Rat monoclonal | 1:100 | BioLegend | 127622 | |
| NeuN | A60 | Rabbit polyclonal | 1:4000 | Millipore | MAB377 | ||
| Iba1 | NCNP24 | Rabbit polyclonal | 1:1000 | Wako | 019-19741 | ||
| CD68 | Gp110 | Rabbit polyclonal | 1:1000 | Invitrogen | PA5-32330 | ||
| PDGFR-α | Goat polyclonal | 1:250 | R & D systems | AF1062 | |||
| PSD-95 | Rabbit polyclonal | 1:200 | Invitrogen | 51-6900 | |||
| Synapsin | Guinea Pig | 1:1000 | Synaptic systems | 106 004 | |||
| Secondary antibody | AF488 | Goat anti-rabbit | 1:500 | Thermo Fisher | A-32731 | ||
| Secondary antibody | AF568 | Goat anti-guinea pig | 1:500 | Thermo Fisher | A-11073 | ||
| Secondary antibody | AF568 | Donkey anti-goat | 1:500 | Thermo Fisher | A-11055 | ||
| Secondary antibody | AF488 | Donkey anti-rabbit | 1:500 | Thermo Fisher | A-21206 | ||
| Secondary antibody | Biotinylated goat anti-rabbit | 1:1000 | Vector Laboratories | BA-1000-1.5 | |||