Table 2.
Primers for serotonin (5-HT) receptors 3, 4, and 7 designed1, cytokines2 and housekeeper genes3
| Primer forward (5′ → 3′) | Primer reverse (5′ → 3′) | References | |
|---|---|---|---|
| 5-HTR3 | 5′-AACTACAAGCCCCTCCAGGT-3′ | 5′-CATGAAAACACTCCTGTCGAA-3′ | This paper |
| 5-HTR4 | 5′-CGTCTGGATTTATGGGGAGA-3′ | 5′-GAACAAGATGACCCCTCTGC-3′ | This paper |
| 5-HTR5 | 5′-GCCACTTCTTCTGCAACGTC-3′ | 5′-CAAGCACACCTTGTCGTCAT-3′ | This paper |
| IL-1α | 5′-GTGCTCAAAACGAAGACGAACC-3′ | 5′-CATATTGCCATGCTTTTCCCAGAA-3′ | [50] |
| IL-1β | 5′-AACGTGCAGTCTATGGAGT-3′ | 5′-GAACACCACTTCTCTCTTCA-3′ | [51] |
| IL-6 | 5′-CTGGCAGAAAACAACCTGAACC-3′ | 5′-TGATTCTCATCAAGCAGGTCTCC-3′ | [51] |
| IL-8 | 5′-GCTCTCTGTGAGGCTGCAGTTC-3′ | 5′-AAGGTGTGGAATGCGTATTTATGC-3′ | [52] |
| IL-10 | 5′-TGAGAACAGCTGCATCCACTTC-3′ | 5′-TCTGGTCCTTCGTTTGAAAGAAA-3′ | [53] |
| IL-12p35 | 5′-AGTTCCAGGCCATGAATGCA-3′ | 5′-TGGCACAGTCTCACTGTTGA-3′ | [53] |
| IL-12p40 | 5′-TTTCAGACCCGACGAACTCT-3′ | 5′-CATTGGGGTACCAGTCCAAC-3′ | [54] |
| TNF-α | 5′-ACTCGGAACCTCATGGACAG-3′ | 5′-AGGGGTGAGTCAGTGTGACC-3′ | [55] |
| IFN-α | 5′-CCCCTGTGCCTGGGAGAT-3′ | 5′-AGGTTTCTGGAGGAAGAGAAGGA-3′ | [56] |
| IFN-γ | 5′-TGGTAGCTCTGGGAAACTGAATG-3′ | 5′-GGCTTTGCGCTGGATCTG-3′ | [53] |
| TGF-β | 5′-CACGTGGAGCTATACCAGAA-3′ | 5′-TCCGGTGACATCAAAGGACA-3′ | [56] |
| β-actin | 5′-CTACGTCGCCCTGGACTTC-3′ | 5′-GATGCCGCAGGATTCCAT-3′ | [57] |
| Cyclophilin | 5′-TGCTTTCACAGAATAATTCCAGGATTTA-3′ | 5′-GACTTGCCACCAGTGCCATTA-3′ | [58] |
| GAPDH | 5′-ACATGGCCTCCAAGGAGTAAGA-3′ | 5′-GATCGAGTTGGGGCTGTGACT-3′ | [58] |
1Designed using Primer Blast® (Bethesda, MD, USA) from sequences XM_003357301.4, NM_001001267.1 and NM_214085.1
2Cytokines = interleukin (IL)-1α, IL1-β, IL-6, IL-8, IL-10, IL-12p35 (IL-12α), IL-12p40 (IL12-β), tumor necrosis factor alpha (TNF-α), interferon alpha and gamma (IFN-α and IFN-γ), transforming growth factor betta (TGF-β)
3β-actin, cyclophilin and glyceraldehyde-3-phosphatedehydrogenase (GAPDH)