Fig. 3.

C215Y mice show decreased number of neurons in deep layers of the cortex. A–C Staining of motor cortex brain sections from E18.5 wt (+ / + , A), C215Y/ + (B), and C215Y/C215Y (C) mice for DAPI (blue), Tbr1 (layer VI, red), and Ctip2 (weak in layer VI, strong in layer V, green). D Quantification of Tbr1-positive cells’ density. E Quantification of Ctip2-positive cells’ density. Data are presented as mean ± SD; n = 3–5 animals; **p < 0.01, ***p < 0.005, significance from the wt is assessed by t-test. Scale bar: 50 µm