Fig. 4.

C215Y mutation in GNAO1 gene affects cortical neural progenitor cells. A–C Staining of brain sections of ventricular and subventricular cortical zones from E18.5 wt (+ / + , A), C215Y/ + (B), and C215Y/C215Y (C) mice for DAPI (blue), Nestin (green) or Tbr2 (red). D Measurement of the ventricular zone thickness. E Quantification of the Nestin staining intensity. F Quantification of Tbr2-positive cells. Data are presented as mean ± SD, n = 3–5 animals. *p < 0.05, **p < 0.01, significance from the wt is assessed by t-test. Scale bar: 20 μm