Fig. 1.

5/6 nephrectomy results in chronic kidney disease. A Timeline of the 5/6 Nx procedure showing right kidney excision followed by left Nx at 2 weeks. B Plasma SDMA, BUN and creatinine (CREAT) levels in sham and nephrectomised (Nx) mice at 12 weeks. Each point represents individual mice (* P < 0.05, *** P < 0.001). C Representative images of kidney from sham or 5/6 Nx mice (scale bar = 1 mm) stained with Masson’s Trichrome (left). Collagen content as measured by colour deconvolution and CKD score (see materials and methods) (right, *** P < 0.001, **** P < 0.0001)