Fig. 5.

CXCL10 blockade reduce cardiac macrophage numbers but did not protect from cardiac dysfunction. A Heatmap showing fold change in cardiac gene expression of chemokines in 5/6 Nx, folate-treated wildtype and folate-treated CCR2^−/− mice (to their respective controls). B Heatmap showing fold change in plasma chemokine levels in 5/6 Nx, folate-treated wildtype and CCR2^−/− mice (to their respective controls). C F4/80⁺ macrophage, lymphocyte, and neutrophil numbers per mg of heart tissue in vehicle-treated mice injected with PBS, or folate-treated mice injected with either anti-CXCL10 antibody or control IgG2a antibody(* P < 0.05, ** P < 0.01). D Representative images showing CD68 staining (scale bar = 50 μm) in vehicle-treated mice injected with PBS, or folate-treated mice injected with either anti-CXCL10 antibody or control IgG2a antibody (* P < 0.05). E) Number of CD68-positve macrophages per mm² of cardiac tissue and proportion of Ki67-positive macrophages in CD68-positive macrophages (grey line) measured in whole heart slice in vehicle-treated mice injected with PBS, or folate-treated mice injected with either anti-CXCL10 antibody or control IgG2a antibody. F) Cardiomyocyte cell areas in hearts of in vehicle-treated mice injected with PBS, or folate-treated mice injected with either anti-CXCL10 antibody or control IgG2a antibody