Table II.
Hepatitis B virus DNA amplification results of 79 donations that were non-discriminated reactive on nucleic acid testing after concentration of viral particles
| Viral particle concentration | Number of PCR reactive samples* | Number of samples confirmed HBV reactive by sequencing | Amplified products | |||
|---|---|---|---|---|---|---|
|
| ||||||
| BCP/PC (276 bp) | Precore/Core (817 bp) | Pre-S/S (1,427 bp) | S (499 bp) | |||
|
| ||||||
| UC | 46 (58.2%) | 43 (54.4%) | 35 (44.3%) | 32 (40.5%) | 25 (31.6%) | 25 (31.6%) |
| PEG precipitation | 34 (43.0%) | 31 (39.2%) | 20 (25.3%) | 18 (22.8%) | 15 (19.0%) | 22 (27.8%) |
| UC+PEG precipitation | 55 (69.6%) | 52 (65.8%) | 35 (44.3%) | 33 (41.8%) | 29 (36.7%) | 29 (36.7%) |
*
At least one reactive PCR per sample.
PCR: polymerase chain reaction; HBV: hepatitis B virus; BCP/PC: basic core promoter/precore; bp: base pairs; UC: ultracentrifugation; PEG: polyethylene glycol.