TABLE 3.
Comparative end point sensitivities of primer sets in multiplex and single-set formats
| Target | Assay format |
CT valuea at the following dilution of DNA:
|
||||
|---|---|---|---|---|---|---|
| Undiluted | 10−1 | 10−2 | 10−3 | 10−4 | ||
| N. meningitidis ctrA | Multiplex | 26.94 | 30.39 | 32.91 | 37.25 | >45.0 |
| Single | 26.85 | 30.07 | 34.25 | 38.73 | >45.0 | |
| H. influenzae bexA | Multiplex | 28.54 | 31.58 | 34.62 | >45.0 | >45.0 |
| Single | 28.49 | 32.07 | 37.96 | >45.0 | >45.0 | |
| S. pneumoniae ply | Multiplex | 29.41 | 32.26 | 35.40 | 36.32 | >45.0 |
| Single | 29.74 | 32.06 | 35.28 | 36.50 | >45.0 | |
a
The CT value is the cycle number at which the measured fluorescent signal exceeds a calculated background threshold identifying amplification of the target sequence; a value of >45.0 is deemed to be negative.