TABLE 5.
Coamplification of gene targets
Template |
CT valuea for the following target gene:
|
||
---|---|---|---|
ctrA | bexA | ply | |
N. meningitidis | 38.00 | ||
H. influenzae | 31.37 | ||
S. pneumoniae | 26.40 | ||
N. meningitidis plus H. influenzae | 39.44 | 31.69 | |
N. meningitidis plus S. pneumoniae | 36.29 | 27.07 | |
H. influenzae plus S. pneumoniae | 31.33 | 26.95 |
CT value is the cycle number at which the measured fluorescent signal exceeds a calculated background threshold identifying amplification of the target sequence.