REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-FGFR1OP (FOP, Rabbit)	Proteintech	Cat#11343-1-AP, PRID: AB_2103362
Anti-ARL13B (Rabbit)	Proteintech	Cat#17711-1-AP, PRID: AB_2060867
Anti-CDH1 (Rat)	Invitrogen	Cat#13-1900, PRID: AB_2533005
Anti-γTubulin (Goat)	Santa Cruz	Cat#sc-7396, PRID: AB_2211262
Anti-p53 (Rabbit)	Proteintech	Cat#10442-1-AP, PRID: AB_2206609
Anti-p21 (Rabbit)	Proteintech	Cat#28248-1-AP, PRID: AB_2881097
Anti-Cleaved Caspase-3 (Rabbit)	Cell Signaling Technology	Cat#9661S, PRID: AB_2341188 9664S
Anti-SOX2 (Rabbit)	Cell Signaling Technology	Cat#23064S, PRID: AB_2714146
Anti-SOX2 (Goat)	R&D	Cat#AF2018, PRID: AB_355110
Anti-SOX9 (Goat)	R&D	Cat#AF3075, PRID: AB_2194160
Anti-TTF-1 (Nkx2.1, Rabbit)	Cell Signaling Technology	Cat#12373S, PRID: AB_2797895
Anti-Prosurfactant Protein C (SFTPC, Rabbit)	Sigma	Cat#AB3786, PRID: AB_91588
Anti-p73 (Rabbit)	Abcam	Cat#ab40658, PRID: AB_776999
Anti-Phospho-p44/42 MAPK (pErk1/2, Rabbit)	Cell Signaling Technology	Cat#9101S, PRID: AB_331646
Anti-Phospho-p44/42 MAPK (pErk1/2, Rabbit)	Cell Signaling Technology	Cat#4370S, PRID: AB_2315112
Anti-p44/42 MAPK (Erk1/2, Rabbit)	Cell Signaling Technology	Cat#4695S, PRID: AB_390779
Anti-Ki67 (Rabbit)	Abcam	Cat#ab15580, RRID: AB_443209
Donkey anti-rat Alexa Fluor 488	Invitrogen	Cat#A-21208, PRID: AB_2535794
Donkey anti-goat Alexa Fluor 647	Invitrogen	Cat#A-21447, PRID: AB_2535864
Donkey anti-rabbit Alexa Fluor 568	Invitrogen	Cat#A-10042, PRID: AB_2534017
Goat Anti-Rabbit IgG HRP.	Jackson ImmunoResearch	Cat#111-035-144, PRID: AB_2307391
Anti-Cdh1 e660	eBioscience	Cat#50-3249-82, PRID: AB_11040003
Anti-Pecam1/CD31 e450	eBioscience	Cat#480311, PRID: AB_10598807
Anti-Ptprc/CD45 e450	eBioscience	Cat#480451, PRID: AB_1518807
Anti-Ter119 e450	eBioscience	Cat#485921, PRID: AB_1518809
Bacterial and Virus Strains
Stable Competent E. coli	NEB	Cat#C3040H
Chemicals, Peptides, and Recombinant Proteins
SCH772984	MedChemExpress	Cat#HY-50846
Doramapimod (BIRB 796)	MedChemExpress	Cat#HY-10320
JNK-IN-8	MedChemExpress	Cat#HY-13319
FluorSave	E.M.D. Millipore Calbiochem	Cat#34-578-920ML
Hoechst 33342	Tdermo Fisher	Cat#H21492
EdU	Tdermo Fisher	Cat#E10187
PhosSTOP	Roche	Cat#04906845001
cOmplete™ EDTA-free Protease Inhibitor Cocktail	Roche	Cat#11873580001
Phenol red-free Leibovitz L-15	Tdermo Fisher	Cat#21083027
Advanced DMEM/F12	Tdermo Fisher	Cat#12634010
Collagenase I	Tdermo Fisher	Cat#17100017
Collagenase II	Tdermo Fisher	Cat#17101015
Dnase I	Tdermo Fisher	Cat#04716728001
Trypsin	Tdermo Fisher	Cat#27250018
FBS	Gibco	Cat#10439-024
BSA	Sigma	Cat#A9418
HEPES	Tdermo Fisher	Cat#15630080
EDTA	Tdermo Fisher	Cat#15575020
RNasin Ribonuclease Inhibitor	Promega	Cat#N2115
Growtd Factor Reduced, Phenol Red-Free Matrigel 10 mg/mL	Corning	Cat#356231
Fgf10	R&D	Cat#6224-FG-025
Fgf9	R&D	Cat#7399-F9-025
EGF	R&D	Cat#2028-EG-200
CHIR99021	Tocris	Cat#4423
Y27632	Tocris	Cat#1254
A8301	Tocris	Cat#2939
Heparin	Sigma	Cat#H3149
Insulin	Roche	Cat#11376497001
Transferrin	Roche	Cat#10652202001
PenStrep	Tdermo Fisher	Cat#15140122
Glutamine	Tdermo Fisher	Cat#25030081
Anti-Anti	Tdermo Fisher	Cat#15240062
Dispase II	Tdermo Fisher	Cat#17105041
Collagenase IV	Tdermo Fisher	Cat#17104019
Papain	Wortdington	Cat#LS003118
Noggin	Peprotech	Cat#10772-456
R-Spondin 1	R&D	Cat#7150-RS-025/CF
TRI reagent	Sigma	Cat#T9424
SuperSignal™ West Pico PLUS Chemiluminescent Substrate	Tdermo Fisher	Cat#PI34577
SuperSignal™ West Femto Maximum Sensitivity Substrate	Tdermo Fisher	Cat#34095
Critical Commercial Assays
PowerUp™ SYBRTM Green Master Mix	Applied Biosystems	Cat#A25742
iScript cDNA syntdesis kit	BioRad	Cat#1708890
RNeasy Mini Kit (250)	Qiagen	Cat#74106
Zymo RNA Clean & Concentrator-5 columns	Zymo Research	Cat#1016
Deposited Data
Raw and processed RNAseq data	Tdis paper	GEO: GSE188585
Raw Western blot images	Tdis paper	DOI:10.17632/tdwstr46bb.1
Experimental Models: Organisms/Strains
Mouse: C57BL/6J	Tde Jackson Laboratory	Jax stock 000664
Mouse: ShhCre: B6.Cg-Shhtm1(E.G.F.P./cre)Cjt/J	Tde Jackson Laboratory	Jax stock 005622
Mouse: Ift88lox: B6.129P2-Ift88tm1Bky/J	Tde Jackson Laboratory	Jax stock 022409
Mouse: Sox2lox:Sox2tm1.1Lan/J	Tde Jackson Laboratory	Jax stock 013093
Mouse: R26tdTomato: Rosa26tm14(CAG-tdTomato)Hze/J	Tde Jackson Laboratory	Jax stock 007914
Mouse: Sox9GFP:Sox9IRES-EGFP	Tde Jackson Laboratory	Jax stock 030137
Mouse ES cell: Cenpjtm1a(EUCOMM.)Wtsi	European Mouse Mutant Cell Repository	EPD0028_7_G05
Mouse: p53 KO: B6.129S2-Trp53tm1Tyj/J	Tde Jackson Laboratory	Jax stock 002101
Mouse: p53LoxP: B6.129P2-Trp53tm1Brn/J	Tde Jackson Laboratory	Jax stock 008462
Mouse: ACTB:FLPe: B6;SJL-Tg(ACTFLPe)9205Dym/J	Tde Jackson Laboratory	Jax stock 005703
Mouse: β-actin-cre: B6N.FVB-Tmem163Tg(ACTB-cre)2Mrt/CjDswJ	Tde Jackson Laboratory	Jax stock 019099
Oligonucleotides
qPCR-Fgf9-F: GGAGTTGGATATACCTCGCCT	Tdis paper	N/A
qPCR-Fgf9-R: TGATCCATACAGCTCCCCCT	Tdis paper	N/A
qPCR-Fgf10-F: GGAGATGTCCGCTGGAGAAG	Tdis paper	N/A
qPCR-Fgf10-R: GCTGTTGATGGCTTTGACGG	Tdis paper	N/A
qPCR-Fgfr2-F: TTTCCTCCGAGATCATCGCC	Tdis paper	N/A
qPCR-Fgfr2-R: ACCATGGGCTACCTGCAATG	Tdis paper	N/A
qPCR-TAp63-F: ATTCAGTTGGAGCAAGGGGG	Tdis paper	N/A
qPCR-TAp63-R: CACGAGAAATGAGCTGGGGT	Tdis paper	N/A
qPCR-DNp63-F: CAGCCTTGACCAGTCTCACT	Tdis paper	N/A
qPCR-DNp63-R: TGTCTTCGCAATCTGGCAGT	Tdis paper	N/A
qPCR-Dolk-F: CAGTGTGGGACCGATACTCCT	(Kopinke et al., 2017)	N/A
qPCR-Dolk-R: CCAAGCAAAGGCATGACCA	(Kopinke et al., 2017)	N/A
qPCR-Hprt-F: CATAACCTGGTTCATCATCGC	(Kopinke et al., 2017)	N/A
qPCR-Hprt-R: TCCTCCTCAGACCGCTTTT	(Kopinke et al., 2017)	N/A
qPCR-Pde12-F: ACCTTTTGGGTGCCAGTAGA	(Kopinke et al., 2017)	N/A
qPCR-Pde12-R: CCAGAGGTCATCTGTCCTTCA	(Kopinke et al., 2017)	N/A
Software and Algoritdms
SDS. Software version 2.3	Tdermo Fisher	https://www.tdermofisher.com/us/en/home/technical-resources/software-downloads/applied-biosystems-7500-real-time-pcr-system.html
QuantStudio™ Design & Analysis 1.3.1	Tdermo Fisher	https://www.tdermofisher.com/us/en/home/global/forms/life-science/quantstudio-3-5-software.html
Prism 8 for Windows	GraphPad Software	www.graphpad.com
CellProfiler version 3.1.9	(McQuin et al., 2018)	https://cellprofiler.org/releases/
R Studio	Rstudio	https://rstudio.com/products/rstudio/download/
R	Tde R Foundation	https://cran.r-project.org/mirrors.html
Galaxy	(Afgan et al., 2018)	https://usegalaxy.org/
FIJI	(Schindelin et al., 2012)	https://imagej.net/Fiji
Zen version 3.1 blue edition	Zeiss	https://www.zeiss.com/microscopy/us/products/microscope-software/zen.html