Fig 3. The A. fumigatus and A. nidulans ΔmtrA mutants are more sensitive to GT.

(A) The A. fumigatus and A. nidulans wild-type and two independent ΔmtrA strains were grown for 48 hours at 37°C on MM in the absence or presence of 30 or 10 μg/ml of GT, respectively. The results are the average of three repetitions ± standard deviation. Statistical analysis was performed using a one-tailed, paired t-test when compared to the control condition (*, p < 0.01). (B) The phylogenetic distribution of MtrA, RglT, GliT and GT biosynthetic gene cluster homologs across 458 fungal genomes. A 4-gene phylogeny of genomes from Eurotiomycetes (shown by the red branches) and Sordariomycetes (shown by the blue branches). For every tip in the phylogeny, the presence of MtrA, RglT, GliT and gliotoxin BGC homologs is depicted using light green, orange, blue, and dark green, respectively; absences are depicted in white. The dark green bar plots depict how many of the 13 Gli genes are present in the gliotoxin BGC homolog. The tip corresponding to the A. fumigatus Af293 genome is indicated by a red dot, and the tip corresponding to the A. nidulans A4 genome is indicated by a maroon dot.