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. 2022 Jan 28;17(1):e0262549. doi: 10.1371/journal.pone.0262549

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© 2022 Suzuki et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — The expression of IL-6 in the culture media of fibroblast and HNSCC cell lines (SAS and FaDu) were evaluated by ELISA. (A) Significantly higher levels of IL-6 were observed in the serum-free culture media of SAS and FaDu cell lines compared with fibroblasts. (B) Expression of IL-6 in culture media of fibroblasts with or without 10 Gy of irradiation was evaluated by ELISA. Results are expressed as fold-changes relative to fibroblasts without irradiation. Expression of IL-6 from fibroblasts was significantly increased with irradiation. The experiments were repeated in triplicate and the data represent the means of three measurements with SD error bars. * P < 0.05.