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. 2020 Nov;9(11):7183–7195. doi: 10.21037/tcr-20-322A

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2020 Translational Cancer Research. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0/.

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Determination of the effect of NS-398 on cell apoptosis and expression levels of cyclooxygenase-2 (COX-2) and survivin in human hepatocellular carcinoma (HCC) cells by Western blotting. (A,B,C,D) Western blots and graphical representations demonstrating COX-2 and survivin expression levels in HCC cells treated with NS-398 and paeonol (Pae), with β-actin as internal control. Data are presented as mean ± standard deviation (SD) (**, P<0.01). Optical density (OD) values versus β-actin presented as fold change of the control values (*, P<0.05; **, P<0.01). (E) Concentration of prostaglandin E2 (PGE2) in treated cells compared with untreated cells measured by ELISA. Data are presented as mean ± SD of three independent experiments (bars = SD) (**, P<0.01). ELISA, enzyme-linked immunosorbent assay.