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. Author manuscript; available in PMC: 2022 Jun 18.
Published in final edited form as: ACS Chem Biol. 2021 May 25;16(6):1079–1089. doi: 10.1021/acschembio.1c00260

Figure 2.

Figure 2.

Induction of the antifungal HSAF and analogs in L. enzymogenes OH11 by fungi. (A) HPLC analysis of HSAF and analogs from OH11 cultured for 48 h in 1/10 TSB supplemented with an increased amount (0%, 0.1%, 0.5%, 1% and 1.5%, w/v) of mycelia preparation of Fusarium graminearum. Traces a-e indicate HPLC of cultures supplemented with 0%, 0.1%, 0.5%, 1% and 1.5% (w/v), respectively, of the mycelia preparation. (B) Quantitative analysis of HSAF and analogs in these cultures. White column, light-gray column, medium-gray column, dark-gray column, and black column indicate cultures supplemented with 0%, 0.1%, 0.5%, 1% and 1.5% (w/v), respectively, of the mycelia preparation. (C) HPLC analysis of HSAF and analogs from OH11 cultured for 48 h in 1/10 TSB supplemented with 1% mycelia preparation of different fungi. Traces a-d indicate HPLC of cultures with no supplement, with Alternaria alternata, with F. graminearum, and with F. verticillioides, respectively. (D) Quantitative analysis of HSAF and analogs in these cultures. White column, light-gray column, medium-gray column, and dark-gray column indicate cultures with no supplement, with A. alternata, with F. graminearum, and with F. verticillioides, respectively. Data are presented as averages of three independent experiments, each conducted in triplicates, with * p < 0.05, ** p < 0.01.