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. 2019 Dec;8(8):2906–2915. doi: 10.21037/tcr.2019.12.16

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2019 Translational Cancer Research. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0/.

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Girdin knockout disrupts mitochondrial membrane potential. (A) and (B) ROS levels were measured as DCF fluorescence by flow cytometry of Girdin-silenced cells following treatment with Z-VAD-FMK. (C) and (D) The change in mitochondrial membrane potential was detected by flow cytometry of Girdin-silenced cells following treatment with Z-VAD-FMK. All experiments were repeated at least three times. Results are shown as means ± SD. ***P<0.001 by one-way ANOVA. a, NC; b, NC + Z-VAD-FMK; c, Girdin shRNA; d, Girdin shRNA + Z-VAD-FMK.