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Infiltrating Treg cells positively correlated with circulating tumor cells and invasiveness phenotype of HCC. (A) Assessment of the purity (left) and apoptosis rate (right) of Treg cells using FACS. (B) Treg cell contents significantly correlated with the numbers of CTCs (P<0.001). (C) Treg cell contents significantly increased in patients with high CTC loads (CTC ≥2 per 7.5 mL) (P<0.001), and patients with vascular invasion exhibited higher Treg cell contents (P=0.038).
