Figure 2.

In vitro effects of infiltrating Treg cells on HCC cells. (A) Migration and invasion potentials of HCC cells were significantly promoted when co-cultured with Treg cells in both Huh7 and 97H cells (Crystal Violet Staining, Magnification, ×100. P<0.050). (B) Effects of infiltrating Tregs on the proliferation potentials of HCC cells detected using CCK8 assays. (C) Effects of infiltrating Treg cells on the cell cycle of HCC cells detected using FACS. Tregs showed a higher proportion of S phase. (D) Cell apoptosis was significantly inhibited when co-cultured with Treg cells.