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. 2020 Dec;9(12):7440–7449. doi: 10.21037/tcr-20-994

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2020 Translational Cancer Research. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0/.

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Detection of HIF-1α and ANXA3 expression in colon cancer and normal colon tissues. (A) qRT-PCR analysis of HIF-1α and ANXA3 mRNA levels in colon cancer (n=60) and normal colon tissues (n=60). (B) Western blot analysis of HIF-1α and ANXA3 protein levels in tumor (T) and normal (N) tissues. The average signal intensity was standardized to β-actin. Data was analyzed using Student’s t-test and was shown as means ± SD of triplicate experiments. *, P<0.05. HIF-1α, hypoxia-inducible factor 1-alpha; ANXA3, annexin A3.